Recombinant Enzymes

Sialate-9-O-acetylesterase

Ordering Numbers:
Est-9-100 (100 mUnits)
Est-9-1000 (1000 mUnits)

Specificity:  Hydrolyzes 9-O-acetyl-groups from glycosidically bound mono- and di-O-acetylated sialic acids

Source: cloned cDNA derived from influenza C virus, expressed in insect cells. Contains no influenza virus.
Unit definition: One Unit is defined as the amount that will release 1 micromole of nitrophenole from 4-nitrophenyl acetate per minute at pH 7.0 and 25oC

 

Use: De-O-acetylates glycosidically bound 9-O-acetylated sialic acids from cell surfaces, glycoproteins, and glycolipids.

 

References:

Vlasak R., Krystal M., Nacht M., Palese P. (1987): The influenza C virus glycoprotein (HE) exhibits receptor-binding (hemagglutinin) and receptor-destroying (esterase) activities. Virology 160, 419-425.
Vlasak, R., T. Muster, et al. (1989). "Influenza C virus esterase: analysis of catalytic site, inhibition, and possible function." J Virol 63(5): 2056-62.
Chatterjee, M., A. K. Chava, et al. (2003). "Identification and characterization of adsorbed serum sialoglycans on Leishmania donovani promastigotes." Glycobiology 13(5): 351-61.
Strasser P., Unger U., Strobl B., Vilas U., Vlasak R. (2004): Recombinant viral sialate-O-acetylesterases. Glycoconj. J. 20, 551-561.
Ghosh, S., S. Bandyopadhyay, et al. (2005). "Interferon gamma promotes survival of lymphoblasts overexpressing 9-O-acetylated sialoglycoconjugates in childhood acute lymphoblastic leukaemia (ALL)." J Cell Biochem 95(1): 206-16.
Erdmann, M., D. Wipfler, et al. (2006). "Differential surface expression and possible function of 9-O- and 7-O-acetylated GD3 (CD60 b and c) during activation and apoptosis of human tonsillar B and T lymphocytes." Glycoconj J 23(9): 627-38.
Mayr, J., T. Haselhorst, et al. (2008). "Influenza C virus and bovine coronavirus esterase reveal a similar catalytic mechanism: new insights for drug discovery." Glycoconj J 25(5): 393-9.

 



 

 

 

SDS-PAGE

SDS-PAGE of purified recombinant influenza C virus esterase, stained with Coomassie.

 


Left lane: purified enzyme (2 microgram)
Right lane: prestained Marker (kDa)

 

Sialate-4-O-acetylesterase

coming soon